Characterizing the AR-TFEB Interactome in SBMA
Summary: Protein-protein interactions are one of the key regulatory steps to normal protein function. Understanding how these protein complexes are altered with mutant AR versus normal AR is key to uncover new mechanisms of mutant AR toxicity in Kennedy’s Disease. In particular, I have previously shown that in Kennedy’s disease, AR can switch between activating or inhibiting a cellular recycling process known as autophagy by altering its interaction with novel co-regulator Transcription Factor E-B (TFEB). In this project, I will define the interacting partners of the AR-TFEB duplex, with the ultimate goal of identifying novel proteins that regulate this molecular switch and have important therapeutic potential for KD.
